Hard drive help

Hi guys this is what I have currently installed on my computer

Drive #1 - SAMSUNG SP2514N (232 GB) This is identified as C
Drive #2 - WDC WD800BB-00JHA0 (74 GB) This is identified as E

CPU #0 AMD Athlon(tm) 64 X2 Dual Core Processor 3600+, 1908 MHz
CPU #1 AMD Athlon(tm) 64 X2 Dual Core Processor 3600+, 1908 MHz

Drive #1 is an IDE ATA.(used as the system drive)
Drive #2 is an IDE ATA setup on a SATA to IDE ATA converter.(nothing on it-Empty)

Everything is seen by the computer, there are no conflicts and no problems.

I want to use Drive #2 as my system drive and keep #1 as storage for game, movies etc.

How do I go about this bearing in mind the following:-

C Drive: Documents and settings 18.8gb
Program files 43gb
Window folder 1.45gb.
Assorted other folders Nvidia settting,Netgear Backup drivers etc etc.

I have Acronis installed so can easily copy across. But what is the best way and what should I take across.

Thanks in anticipation